文章摘要
周宋汇,沈培亮,汪瑞辰.沙利度胺调控PD-L1抑制HT-29结肠癌细胞的作用及机制研究[J].药学与临床研究,2020,28(3):161~164
沙利度胺调控PD-L1抑制HT-29结肠癌细胞的作用及机制研究
Effect and Mechanism of Thalidomide on Inhibition of HT-29 Colon Cancer Cells by Regulation of PD-L1 Expression
投稿时间:2020-02-03  修订日期:2020-06-17
DOI:
中文关键词: 沙利度胺  PD-L1  结肠癌细胞  免疫检查点
英文关键词: Thalidomide  PD-L1  Colon cancer  Immune checkpoint
基金项目:
作者单位E-mail
周宋汇 江苏省肿瘤医院
伊尔瑞生物科技江苏有限公司 
zsh_511@163.com 
沈培亮 伊尔瑞生物科技江苏有限公司  
汪瑞辰 江苏省肿瘤医院 raychon@126.com 
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中文摘要:
      目的:探讨沙利度胺对HT-29结肠癌细胞的抑制作用及其机制。方法:CCK-8检测沙利度胺对HT-29结肠癌细胞增殖的作用;流式细胞术检测沙利度胺对HT-29结肠癌细胞凋亡的作用和对HT-29结肠癌细胞PD-L1表达的作用;Western Blot检测沙利度胺对HT-29结肠癌细胞c-Myc、STAT3、HIF-1蛋白表达的影响。结果:沙利度胺各浓度(40、80、160、320 μmol·L-1)明显抑制HT-29结肠癌细胞的增殖;沙利度胺各浓度(20、40、80 μmol·L-1)明显促进HT-29结肠癌细胞的凋亡,明显减少HT-29结肠癌细胞表面PD-L1的表达,明显减少HT-29结肠癌细胞c-Myc、STAT3、HIF-1蛋白的表达。结论:沙利度胺对HT-29结肠癌细胞具有抑制作用,其机制可能与减少PD-L1表达及抑制癌细胞上游c-Myc、STAT3、HIF-1信号分子表达有关。
英文摘要:
      Objective: To study the effect and mechanism of thalidomide on inhibition of HT-29 colon cancer cells. Methods: CCK-8 was used to study the effect of thalidomide on the proliferation of cancer cell line HT-29. Flow cytometry was used to study the effects of thalidomide on the apoptosis of cancer cell line HT-29 and on the expression of PD-L1 on the membrane of cancer cell line HT-29. Western blot was used to study the effect of thalidomide on the protein expression of c-Myc, STAT3 and HIF-1 of cancer cell line HT-29. Results: Thalidomide (40, 80, 160 and 320 μmol·L-1) inhibited the proliferation of cancer cell line HT-29. Thalidomide (20, 40 and 80 μmol·L-1) induced the apoptosis of cancer cell line HT-29. Thalidomide (20, 40 and 80 μmol·L-1) reduced the expression of PD-L1 on the membrane of cancer cell line HT-29. Thalidomide (20, 40 and 80 μmol·L-1) reduced the protein expression of c-Myc, STAT3 and HIF-1 of cancer cell line HT-29. Conclusion: Thalidomide may inhibit the proliferation of HT-29 colon cancer cells by reducing membrane PD-L1 and the expression of c-Myc, STAT3 and HIF-1.
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