文章摘要
徐佳雯,李尚,张彦卓,胡雅君,张斐.HPLC法同时测定地西泮直肠凝胶中地西泮、 苯甲酸、苯甲醇含量[J].药学与临床研究,2020,28(2):97~100
HPLC法同时测定地西泮直肠凝胶中地西泮、 苯甲酸、苯甲醇含量
Simultaneous determination of Diazepam, Benzoic Acid and Benzyl Alcohol in Diazepam Rectal Gel by HPLC
投稿时间:2019-09-02  修订日期:2020-04-23
DOI:
中文关键词: 地西泮  苯甲酸  苯甲醇  含量  高效液相色谱法
英文关键词: Diazepam  Benzoic acid  Benzyl alcohol  Content determination  HPLC
基金项目:
作者单位E-mail
徐佳雯 徐州医科大学 244248273@qq.com 
李尚 徐州医科大学  
张彦卓 徐州医科大学  
胡雅君 江苏恩华药业股份有限公司  
张斐 徐州医科大学 zhangfeily@126.com 
摘要点击次数: 1013
全文下载次数: 965
中文摘要:
      目的:建立了同时测定地西泮直肠凝胶中地西泮、苯甲酸、苯甲醇含量的高效液相色谱方法。方法:色谱柱为Agilent Eclipse Plus C18(4.6 mm × 150 mm,3.5 μm),以甲醇-0.05 mol·L-1 乙酸铵缓冲溶液(pH 4.0)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长254 nm,柱温30 ℃,进样量10 μL。结果:地西泮、苯甲酸、苯甲醇分离度均大于1.5,地西泮在0.010~0.200 mg·mL-1范围内线性关系良好(r≥0.999 9);苯甲酸在0.030~0.600 mg·mL-1范围内线性关系良好(r≥0.999 9);苯甲醇在0.0718~1.436 mg·mL-1范围内线性关系良好(r≥0.999 9);且地西泮、苯甲酸、苯甲醇的平均回收率均在98%~101%,RSD均小于2.0%(n=9)。结论:本法简单准确可靠,可用于地西泮直肠凝胶中地西泮、苯甲酸、苯甲醇的含量测定。
英文摘要:
      Objective: A method for the simultaneous determination of diazepam, benzoic acid and benzyl alcohol in diazepam rectal gels was established. Methods: The analysis was performed by HPLC with an Agilent Eclipse Plus C18 column (4.6 mm × 150 mm, 3.5 μm) and a gradient elution of mobile phase of methanol -0.05 mol·L-1 ammonium acetate buffer (pH 4.0) at 1.0 mL·min-1, the detection wavelength was 254 nm and the column temperature was 30 ℃. Results: The resolutions of benzoic acid, benzyl alcohol and diazepam were over 1.5. The linear ranges were 0.030-0.600 mg·mL-1 (r≥0.999 9), 0.0718-1.436 mg·mL-1(r≥0.999 9) and 0.010-0.200 mg·mL-1(r≥0.999 9) for benzoic acid, benzyl alcohol and diazepam, respectively; The average recoveries of benzoic acid, benzyl alcohol and diazepam were between 98% and 101%, and the RSDs were within 2.0% (n=9). Conclusion: The method is simple, accurate and reliable, and can be used for the determination of diazepam, benzoic acid and benzyl alcohol in the diazepam rectal gels.
查看全文   查看/发表评论  下载PDF阅读器
关闭