文章摘要
刘力亨.五味子丙素抑制脂多糖诱导心肌细胞的炎症反应与 细胞焦亡的作用及机制研究[J].药学与临床研究,2020,28(2):81~85
五味子丙素抑制脂多糖诱导心肌细胞的炎症反应与 细胞焦亡的作用及机制研究
Effect and Mechanism of Schisandrin C on Lipopolysaccharide-induced Myocardial Inflammatory Response and Pyroptosis
投稿时间:2019-10-14  修订日期:2020-04-23
DOI:
中文关键词: 脓毒症  脂多糖  五味子丙素  细胞焦亡  GSDMD
英文关键词: Sepsis  Lipopolysaccharide (LPS)  Schisandrin C (SchC)  Pyroptosis  GSDMD
基金项目:
作者单位E-mail
刘力亨 南京医科大学附属儿童医院药学部 380251774@qq.com 
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中文摘要:
      目的:探究五味子丙素(SchC)对脂多糖(LPS)诱导心肌细胞HL-1炎症反应与细胞焦亡的影响。方法:培养小鼠心肌细胞HL-1,将其分为空白对照组、模型组(LPS)、LPS+SchC组。SchC预处理1 h后,分别以LPS刺激24 h或48 h,ELISA法提取细胞培养液上清液,检测细胞因子IL-1β、HMGB1和TNF-α的分泌,MTT法收集细胞检测细胞活力,Western Blot检测cleaved-Caspase1、GSDMD-N和NLRP3的表达;将HL-1细胞分为空白对照组、模型组(LPS)、LPS+siGSDMD(GSDMD siRNA)组、LPS+siGSDMD+SchC组,给药预处理1 h后,LPS刺激24 h,收集细胞培养液上清液,ELISA法检测IL-1β的分泌,MTT法检测细胞活力。结果:与LPS组比较,LPS+SchC组细胞活力得到改善(P<0.05),IL-1β、HMGB1和TNF-α的分泌均显著降低(P<0.05);Western Blot结果表明,与LPS组比较,LPS+SchC组的cleaved-Caspase1、GSDMD-N段和NLRP3蛋白水平显著降低(P<0.05);ELISA和MTT结果表明,与LPS组比较,LPS+siGSDMD组IL-1β的分泌显著降低(P<0.05)且细胞活力显著得到改善(P<0.01),然而LPS+siGSDMD组与LPS+siGSDMD+SchC组的IL-1β分泌水平和细胞活力并无显著性差异。结论:五味子丙素能有效缓解LPS诱导HL-1的炎症反应和细胞焦亡。
英文摘要:
      Objective: To investigate the effects of schisandrin C (SchC) on inflammatory response and pyroptosis induced by lipopolysaccharide (LPS) in HL-1 cells. Methods: HL-1 cells were cultured and divided into blank control group, model group (LPS) and LPS+SchC group. HL-1 cells were stimulated with LPS for 24 hours or 48 hours, with or without pretreatment of SchC for 1 hour, respectively, as indicated. The secretions of cytokines (IL-1β, HMGB1 and TNF-α) were detected by ELISA. Cell viabilities were measured by MTT. The expressions of cleaved-caspase 1, GSDMD-N and NLRP3 were detected by Western blot. HL-1 cells were further divided into blank control group, model group (LPS), LPS+siGSDMD group, LPS+siGSDMD+SchC group under 1 hour of pretreatment with SchC and 24 hours of stimulation with LPS as indicated. Results: Compared with those in the LPS group, the cell viability was improved and the secretions of IL-1β, HMGB1 and TNF-α and the protein levels of cleaved-Caspase-1, GSDMD-N and NLRP3 were decreased significantly (P<0.05) in the LPS+SchC group. Compared with those in the LPS group, the secretion of IL-1β was significantly decreased and the cell viability was improved in the LPS+siGSDMD group; However, there was no significant difference in IL-1β secretion and cell viability between the LPS+siGSDMD group and the LPS+siGSDMD+SchC group. Conclusion: SchC can effectively alleviate LPS-induced inflammation and pyroptosis of HL-1 cells.
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