文章摘要
葛玉松.HPLC法同时测定丁细牙痛胶囊中细辛脂素、原儿茶醛和原儿茶酸的含量[J].药学与临床研究,2019,27(4):261~263
HPLC法同时测定丁细牙痛胶囊中细辛脂素、原儿茶醛和原儿茶酸的含量
Determination of Asarinin, Protocatechuic Aldehyde and Protocatechuic Acid in Dingxi Yatong Capsules by HPLC
投稿时间:2019-01-14  修订日期:2019-08-09
DOI:
中文关键词: HPLC法  丁细牙痛胶囊  细辛脂素  原儿茶醛  原儿茶酸
英文关键词: HPLC  Dingxi Yatong Capsules  Asarinin  Protocatechuic aldehyde  Protocatechuic acid
基金项目:
作者单位E-mail
葛玉松 盐城市食品药品监督检验中心 356345642@qq.com 
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中文摘要:
      目的:建立高效液相色谱法测定丁细牙痛胶囊中细辛脂素、原儿茶醛、原儿茶酸含量。方法:采用高效液相色谱法。色谱柱:Capcell Pak MG Ⅱ C18(4.6 mm × 150 mm, 5 μm);流动相:乙腈-1%冰醋酸溶液,梯度洗脱;检测波长:287 nm;柱温:35 ℃;流速:1 mL·min-1;进样量:10 μL。结果:细辛脂素在0.015 25 ~ 0.152 5 mg·mL-1范围内线性关系良好(r=0.999 9),平均加样回收率为98.01%, RSD为1.10%(n=6);原儿茶酸在0.020 88 ~ 0.208 8 mg·mL-1范围内线性关系良好(r=0.999 9),平均加样回收率为99.34%,RSD为1.48%(n=6);原儿茶醛在0.010 52~0.105 2 mg·mL-1范围内线性关系良好(r=0.999 9),平均加样回收率为101.48%,RSD为1.20%(n=6)。结论:本方法简便可靠,重现性好,适用于丁细牙痛胶囊的质量控制。
英文摘要:
      Objective: To develop an HPLC method for the quantification of asarinin, protocatechuic aldehyde and protocatechuic acid in Dingxi Yatong Capsules. Methods: Separation was carried out using Capcell Pak MG Ⅱ C18 (4.6 mm × 150 mm, 5 μm) with mobile phase of acetonitrile and 1% glacial acid in gradient elution. The detection wavelength was 287 nm. The flow rate was 1 mL·min-1. Results: The linear concentration range of asarinin was 0.015 25-0.152 5 mg·mL-1 (r=0.999 9), the average recovery was 98.01% (RSD=1.10%, n=6). The linear concentration range of protocatechuic acid was 0.020 88-0.208 8 mg·mL-1 (r=0.999 9), the average recovery was 99.34% (RSD=1.48%, n=6). The linear concentration range of protocatechuic aldehyde was 0.010 52-0.105 2 mg·mL-1 (r=0.999 9) the average recovery was 101.48% (RSD=1.20%, n=6). Conclusion: The method is specific, rapid and sensitive, which is suitable for the quality control of asarinin, protocatechuic aldehyde and protocatechuic acid in Dingxi Yatong Capsules.
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