文章摘要
许倩倩,包贝华,张丽,赵学龙,谈瑄忠,李文.响应面法优化乌蔹莓的提取工艺[J].药学与临床研究,2019,27(4):257~260
响应面法优化乌蔹莓的提取工艺
Optimization of Extraction Process for Cayratia Japonica Ointment by the Response Surface Method
投稿时间:2019-03-01  修订日期:2019-08-10
DOI:
中文关键词: 乌蔹莓  响应面法  醇提工艺  木犀草素
英文关键词: Cayratia japonica  Response surface method  Alcohol extraction process  Luteolin
基金项目:南京市第五批老中医药专家学术经验继承项目;南京药学会-常州四药医院药学科研基金项目(2016YX020);南京市医学科技发展资金立项项目(YKK16294);南京市中医药转化医学基地项目(ZHCX201806)
作者单位E-mail
许倩倩 南京中医药大学 xuqianqian0617@163.com 
包贝华 南京中医药大学  
张丽 南京中医药大学  
赵学龙 南京市中医院 zhaoxuelong301@163.com 
谈瑄忠 南京市中医院  
李文 南京市中医院  
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中文摘要:
      目的:利用Box-Behnken响应面法优化乌蔹莓软膏原料的最佳醇提工艺。方法:以干浸膏得率和木犀草素含量作为评价指标,通过响应面法优化工艺,采用综合评分法作评价指标,并进行二项式方程拟合,预测最佳工艺。结果:对指标影响最大的是醇倍量,其次为提取时间,影响最小的为乙醇浓度。最佳醇提工艺条件为:醇倍量12倍;提取时间2.5 h;乙醇浓度70%。3次验证试验的木犀草素平均含量为0.32 mg·g-1;综合得分为0.98,与预测值的相对误差为2.2%。结论:响应面法优化的提取干浸膏工艺高效稳定,木犀草素提取率较高,为乌蔹莓软膏的开发奠定了基础。
英文摘要:
      Objective: To optimize the alcohol extraction process for raw material of Cayratia japonica ointments by the Box-Behnken response surface method. Methods: The yield of dry extracts and the content of luteolin were used as the evaluation indexes. The optimal process was predicted by single factor investigation and Box-Behnken response surface method through comprehensive scoring and binomial equation fitting. Results: The most significant factor on the indexes was alcohol ratio, followed by extraction time, and the least was ethanol concentration. The optimum conditions of ethanol extraction were as follows: the ratio of alcohol was 12 times, the extraction time was 2.5 h, and the concentration of ethanol was 70%. The average content of luteolin was 0.32 mg·g-1. The overall score was 0.98 and the relative error was 2.2%. Conclusion: The extraction process optimized by the Box-Behnken response surface method is efficient and stable with high extraction rate of luteolin, which lays a foundation for the development of Cayratia japonica ointments.
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