文章摘要
周梦玲,袁厚宇,吴素玲.麦冬多糖对脂多糖诱导巨噬细胞损伤的保护作用及机制研究[J].药学与临床研究,2019,27(6):416~420
麦冬多糖对脂多糖诱导巨噬细胞损伤的保护作用及机制研究
Protective Effects and Mechanism of Ophiopogon Japonicus Polysaccharide on Lipopolysaccharide-induced Macrophage Damage
投稿时间:2019-06-23  修订日期:2019-12-07
DOI:
中文关键词: 麦冬多糖  巨噬细胞  细胞损伤  NF-κB
英文关键词: Ophiopogon japonicus polysaccharide  Macrophages  Cell damage  NF-κB
基金项目:南京市卫生和计划生育委员会一般项目(YKK17154)
作者单位E-mail
周梦玲 南京中医药大学 327929604@qq.com 
袁厚宇 南京中医药大学  
吴素玲 南京市第一医院 296094815@qq.com 
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中文摘要:
      目的:研究麦冬多糖对脂多糖(LPS)诱导巨噬细胞损伤的保护作用并探讨其可能机制。方法:采用不同浓度的LPS(0、1、2、4、8、16、32、64、128 μg·mL-1)和麦冬多糖(0、5、10、50、100、500、1000 μg·mL-1)分别处理细胞6 h和24 h,CCK-8法筛选造模剂及药物浓度;ELISA法检测上清液中IL-1β、IL-6、TNF-α的含量,试剂盒检测SOD活力和MDA含量,Western blot检测NF-κB信号通路的相关蛋白表达。结果:当LPS浓度在8 μg·mL-1时,巨噬细胞活力明显下降,而麦冬多糖各剂量对巨噬细胞活力无显著影响,故选取8 μg·mL-1 LPS为造模剂浓度,50、100、500 μg·mL-1为麦冬多糖低、中、高剂量;与对照组相比,模型组上清液中TNF-α、IL-6、IL-1β、MDA水平均显著升高,SOD活性显著降低,TLR4、MyD88表达和IκBα、p65磷酸化水平显著升高,IκBα蛋白表达水平显著降低;采用麦冬多糖低、中、高剂量预处理后,麦冬多糖各剂量组巨噬细胞活力升高,上清液中IL-1β、IL-6、TNF-α水平下降,MDA水平降低,SOD活力提高,IκBα表达升高,TLR4、MyD88的表达和IκBα、p65的磷酸化水平下降。结论:麦冬多糖能够抑制NF-κB信号通路的激活,降低氧化应激反应,从而减轻巨噬细胞的损伤,减少炎性因子的分泌。
英文摘要:
      Objective: To study the protective effects of Ophiopogon japonicus polysaccharides on lipopolysaccharide (LPS)-induced macrophage injury and explore their possible mechanism. Methods: Cells were treated with different concentrations of LPS (0, 1, 2, 4, 8, 16, 32, 64 or 128 μg·mL-1) or Ophiopogon japonicus polysaccharides (0, 5, 10, 50, 100, 500 or 1000 μg·mL-1) for 6 h or 24 h, respectively. CCK-8 was used to screen the concentration of the modeling LPS and the concentrations of the drug polysaccharides. The levels of IL-1β, IL-6 and TNF-α in the supernatant were detected by ELISA and the SOD activities and MDA contents were detected by corresponding kits. Western blot was used to detect the expression of related proteins in NF-κB signaling pathway. Results: When the concentration of LPS was over 8 μg·mL-1, the activity of macrophages decreased significantly, while the dose of Ophiopogon japonicus polysaccharides had no significant effect on the activity of macrophages. Therefore, LPS at 8 mg·mL-1 was selected for modeling. The low, medium and high doses of Ophiopogon japonicus polysaccharides were set at 50, 100 and 500 μg·mL-1. Compared with the control group, the levels of TNF-α, IL-6, IL-1β and MDA in the supernatant of the model group were significantly increased, the activity of SOD was significantly decreased, the expression of TLR4 and MyD88 and the phosphorylation of IκBα and p65 were significantly increased, and the expression of IκBα was significantly decreased. After pretreatment with low, medium and high doses of Ophiopogon japonicus polysaccharides, the activity of macrophages increased, the levels of IL-1β, IL-6 and TNF-α in the supernatant decreased, the level of MDA decreased, the activity of SOD increased, the expression of IκBα increased, the expression of TLR4 and MyD88 and the phosphorylation level of IκBα and p65 decreased. Conclusion: Ophiopogon japonicus polysaccharides can inhibit the activation of NF-κB signaling pathway and oxidative stress, thereby reducing macrophage damage and reducing the secretion of inflammatory factors.
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