文章摘要
周洁,汤维维,陈君.基于UPLC-QTOF-MS/MS法的茅苍术与 北苍术化学成分分析[J].药学与临床研究,2020,28(5):321~328
基于UPLC-QTOF-MS/MS法的茅苍术与 北苍术化学成分分析
A UPLC-QTOF-MS/MS Method for the Characterization of Chemical Constituents of Atractylodes lancea (Thunb.) DC. and Atractylodes chinensis (DC.) Koidz
投稿时间:2020-05-03  修订日期:2020-10-19
DOI:
中文关键词: UPLC-QTOF-MS/MS  苍术  化学成分  化合物鉴定
英文关键词: UPLC-QTOF-MS/MS  Atractylodis Rhizoma  Chemical constituents  Compound identification
基金项目:国家重点研发计划(2018YFC1704501)
作者单位E-mail
周洁 中国药科大学 中药学院生药学系 zhoujie0578@163.com 
汤维维 中国药科大学 中药学院生药学系  
陈君 中国药科大学 中药学院生药学系 chenj2002cpu@126.com 
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中文摘要:
      目的:建立超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS/MS)联用方法,对茅苍术与北苍术的75%甲醇提取物中的化学成分进行定性分析。方法:采用Agilent Infinity Lab Poroshell 120EC-C18(2.1 mm × 100 mm,2.7 μm)色谱柱分离,以0.1%甲酸溶液(A)-乙腈(B)为流动相进行梯度洗脱,流速为0.3 mL·min-1,柱温25 ℃,进样体积1 μL;检测质谱采用四极杆-飞行时间串联质谱系统(电喷雾离子源ESI),正、负离子模式下采集数据,全扫描的质量扫描范围m/z 50~1500。结果:根据UPLC-QTOF-MS/MS提供的各成分的液相色谱保留时间、精确分子质量和特征碎片离子信息,并结合对照品及相关文献数据比对,从茅苍术与北苍术中均鉴定出78个化学成分,包括16个萜类化合物、10个聚乙烯炔类化合物、25个有机酸类化合物、17个糖苷类化合物、10个其它类化合物。结论:该方法可以快速、准确地对苍术药材中的化学成分进行定性表征,可为苍术的物质基础研究和质量控制提供实验依据。
英文摘要:
      Objective: To analyze and identify the chemical constituents of 75% methanol extracts of Atractylodes lancea (Thunb.) DC. and Atractylodes chinensis (DC.) Koidz. by using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-QTOF-MS/MS). Methods: The UPLC was conducted with an Agilent Infinity Lab Poroshell 120EC-C18 column (2.1 × 100 mm, 2.7 μm) and a gradient solvent system consisting of water containing 0.1% formic acid (A) and acetonitrile (B). The flow rate was 0.3 mL·min-1, the temperature of column was maintained at 25 ℃ and the injection volume was 1 μL. The QTOF-MS/MS and electrospray ionization-(ESI) source was applied for the qualitative analysis under the positive and negative ion modes and the full mass scan range of m/z 50-1500. Results: A total of 78 compounds, including 16 terpenoids, 10 polyacetylene compounds, 25 organic acids, 17 glucosides and 10 other compounds, were identified from Atractylodes lancea (Thunb.) DC. and Atractylodes chinensis (DC.) Koidz. by comparing their retention time, accurate mass and diagnostic product ions with those of reference substances and literature data. Conclusion: The method established is rapid and accurate for elucidating the chemical constituents of Atractylodis Rhizoma and can be used for their quality control.
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