文章摘要
张晓芸,张建茹,崔萍,昝艳楠,曹庆丰,丁逸梅.LC-MS/MS法测定注射剂中来自生产管路中的 PFOA和PFOS的迁移量[J].药学与临床研究,2020,28(5):329~332
LC-MS/MS法测定注射剂中来自生产管路中的 PFOA和PFOS的迁移量
Determination of the Migration of PFOA and PFOS from Manufacture Pipeline in Injections by LC-MS/MS
投稿时间:2020-05-14  修订日期:2020-10-20
DOI:
中文关键词: 液相色谱-质谱联用技术  全氟辛酸(PFOA)  全氟辛基磺酸(PFOS)  迁移  生产管路
英文关键词: LC-MS/MS  Pentadecafluorooctanoic acid (PFOA)  Perfluorooctane sulfonate (PFOS)  Migration  Manufacture pipeline
基金项目:
作者单位E-mail
张晓芸 南京工业大学江苏省药物研究所 yunlovers@163.com 
张建茹 南京工业大学药学院  
崔萍 南京工业大学江苏省药物研究所  
昝艳楠 南京工业大学江苏省药物研究所  
曹庆丰 南京工业大学江苏省药物研究所  
丁逸梅 南京工业大学药学院  
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中文摘要:
      目的:建立LC-MS/MS法测定注射剂制备时迁移至管路中的全氟辛酸(PFOA)和全氟辛基磺酸(PFOS)的含量。方法:采用色谱柱Kromasil C8(150 mm×3.0 mm,5 μm),以0.01 mol·mL-1乙酸铵-乙腈(50∶50,v/v)为流动相。采用电喷雾离子源(ESI)、负离子模式检测方式进行定量分析。结果:PFOA和PFOS在5~50 ng·mL-1范围内线性良好(r>0.999 0);PFOA回收率在90.92%~119.2%,PFOS回收率在80.93%~104.9%。结论:采用LC-MS/MS法测定注射剂中来自生产管路的PFOA和PFOS的含量,通过标准加入法能够消除注射剂复杂基体效应对PFOA和PFOS测定的影响,简便、快速地测定PFOA和PFOS的迁移量。
英文摘要:
      Objective: To establish an LC-MS/MS method for the determination of PFOS and PFOA in injections from manufacture pipeline. Methods: A Kromasil C8 column (150 mm×3.0 mm, 5 μm) was applied for LC with 0.01 mol·mL-1 ammonium acetate-acetonitrile (50∶50, v/v) as the mobile phase. MS/MS quantitative analysis was carried out by using the electric spray ion source (ESI) and the negative ion mode detection method. Results: PFOS and PFOA in the coated stopper of QT liposome injections were linear in the range of 5-50 ng·mL-1 (r>0.999); the recovery of PFOA was between 90.92% and 119.2%, the recovery of PFOS was between 80.93% and 104.9%. Conclusion: The established LC-MS/MS method can determine PFOS and PFOA in injections from manufacture pipeline. The matrix effect from injection drugs can be removed by using a standard addition method. The method is accurate, easy and effective.
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