| 张晓芸,张建茹,崔萍,昝艳楠,曹庆丰,丁逸梅.LC-MS/MS法测定注射剂中来自生产管路中的 PFOA和PFOS的迁移量[J].药学与临床研究,2020,28(5):329~332 |
| LC-MS/MS法测定注射剂中来自生产管路中的 PFOA和PFOS的迁移量 |
| Determination of the Migration of PFOA and PFOS from Manufacture Pipeline in Injections by LC-MS/MS |
| 投稿时间：2020-05-14 修订日期：2020-10-20 |
| DOI： |
| 中文关键词: 液相色谱-质谱联用技术 全氟辛酸（PFOA） 全氟辛基磺酸（PFOS） 迁移 生产管路 |
| 英文关键词: LC-MS/MS Pentadecafluorooctanoic acid (PFOA) Perfluorooctane sulfonate (PFOS) Migration Manufacture pipeline |
| 基金项目: |
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| 中文摘要: |
| 目的：建立LC-MS/MS法测定注射剂制备时迁移至管路中的全氟辛酸（PFOA）和全氟辛基磺酸（PFOS）的含量。方法：采用色谱柱Kromasil C8（150 mm×3.0 mm，5 μm），以0.01 mol·mL-1乙酸铵-乙腈（50∶50，v/v）为流动相。采用电喷雾离子源（ESI）、负离子模式检测方式进行定量分析。结果：PFOA和PFOS在5~50 ng·mL-1范围内线性良好（r>0.999 0）；PFOA回收率在90.92%~119.2%，PFOS回收率在80.93%~104.9%。结论：采用LC-MS/MS法测定注射剂中来自生产管路的PFOA和PFOS的含量，通过标准加入法能够消除注射剂复杂基体效应对PFOA和PFOS测定的影响，简便、快速地测定PFOA和PFOS的迁移量。 |
| 英文摘要: |
| Objective: To establish an LC-MS/MS method for the determination of PFOS and PFOA in injections from manufacture pipeline. Methods: A Kromasil C8 column (150 mm×3.0 mm, 5 μm) was applied for LC with 0.01 mol·mL-1 ammonium acetate-acetonitrile (50∶50, v/v) as the mobile phase. MS/MS quantitative analysis was carried out by using the electric spray ion source (ESI) and the negative ion mode detection method. Results: PFOS and PFOA in the coated stopper of QT liposome injections were linear in the range of 5-50 ng·mL-1 (r>0.999); the recovery of PFOA was between 90.92% and 119.2%, the recovery of PFOS was between 80.93% and 104.9%. Conclusion: The established LC-MS/MS method can determine PFOS and PFOA in injections from manufacture pipeline. The matrix effect from injection drugs can be removed by using a standard addition method. The method is accurate, easy and effective. |
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