文章摘要
周亚菁,沈于兰.UPLC法测定维C银翘片中6种有效成分的含量[J].药学与临床研究,2021,29(2):26~28
UPLC法测定维C银翘片中6种有效成分的含量
Determination of Six Constituents in Vitamin C Yinqiao Tablets by UPLC
投稿时间:2020-06-21  修订日期:2021-04-13
DOI:
中文关键词: 维C银翘片  UPLC法  维生素C  对乙酰氨基酚  绿原酸  甘草苷  连翘苷  牛蒡苷
英文关键词: Vitamin C Yinqiao Tablets  UPLC  Vitamin C  Acetaminophen  Chlorogenic acid  Liquiritin  Forsythin  Arctiin
基金项目:
作者单位E-mail
周亚菁 无锡市药品安全检验检测中心 513729364@qq.com 
沈于兰 无锡市药品安全检验检测中心  
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中文摘要:
      目的:建立UPLC法同时测定维C银翘片中维生素C、对乙酰氨基酚、绿原酸、甘草苷、连翘苷、牛蒡苷的含量。方法:色谱柱:Thermo BDS Hypersil C18(4.6 mm × 100 mm,3.0 μm);流动相:水(A),0.1%甲酸-乙腈(B),梯度洗脱;检测波长:280 nm;柱温:25 ℃;流速:0.2 mL·min-1;进样量:2 μL。结果:所测6种成分在测定范围内均有良好的线性关系(r ≥0.9999),平均回收率为93.53% ~ 101.60%。结论:本法分离效果好,灵敏度高,重现性良好,优化了该药的质控方法。
英文摘要:
      Objective: To establish a method for simultaneously determining six constituents (vitamin C, acetaminophen, chlorogenic acid, liquiritin, forsythin and arctiin) in Vitamin C Yinqiao Tablets by UPLC. Methods: The separation was performed on a Thermo BDS Hypersil C18 (4.6 mm × 100 mm, 3.0 μm) column with water and acetonitrile-0.1% formic acid eluting at 0.2 mL·min-1 in a gradient manner at 25 ℃. The UV detection wavelength was set at 280nm. Results: The calibration curves of the six constituents (vitamin C, acetaminophen, chlorogenic acid, liquiritin, forsythin and arctiin) were linear in their measurement concentration ranges (r≥0.9999). The average recoveries were from 93.53% to 101.60% (n=9). Conclusions: The method is rapid and sensitive with good reproducibility, which supplements the quality control methods of Vitamin C Yinqiao Tablets.
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