文章摘要
林玲,吴勤研,宋熙薇.基于网络药理学的康艾注射液抗非小细胞肺癌作用机制分析[J].药学与临床研究,2021,29(2):6~11
基于网络药理学的康艾注射液抗非小细胞肺癌作用机制分析
Mechanism of Kangai Injection in the Treatment of Non-Small-Cell Lung Cancer Based on Network Pharmacology
投稿时间:2020-07-29  修订日期:2021-03-26
DOI:
中文关键词: 琥珀酸曲格列汀  高效液相色谱  校正因子  有关物质
英文关键词: Kangai injection  Network pharmacology  Non-small-cell lung cancer  Mechanism
基金项目:
作者单位E-mail
林玲 东部战区总医院 llgg1987@163.com 
吴勤研 东部战区总医院  
宋熙薇 中国人民解放军东部战区总医院药品科 sxw_anym@163.com 
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中文摘要:
      目的:采用网络药理学方法探讨康艾注射液抗非小细胞肺癌作用机制。方法:依托中药系统药理学分析平台(TCMSP)检索康艾注射液中3味中药化学成分和相关作用靶点。将靶点上传到UniProtKB软件,得到的基因与人类基因组注释数据库(Genecards)对映。运用Cytoscape3.7.0软件构建化合物-靶点网络,借助STRING10.5软件构建蛋白互作(protein-protein interaction,PPI)网络,采用DAVID6.8软件进行基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)生物通路富集分析。结果:通过筛选得到23个化合物,对映的靶点有100个。PPI网络包含93个靶点,关键靶点涉及IL6、TNF、JUN、MYC、CASP3、MAPK8和PTGS2等。GO条目43个,其中生物过程相关的条目有32个,分子功能相关的条目6个,细胞组成相关的条目有5个。KEGG通路5条,涉及癌症通路、IL-7通路、肿瘤坏死因子通路、雌激素信号通路和NF-Kappa B信号通路。结论:本研究初步阐释了康艾注射液抗非小细胞肺癌的基本药理作用及其机制,为进一步深入研究提供了参考。
英文摘要:
      Objective: To investigate the mechanism of Kangai Injection against non-small-cell lung cancer (NSCLC) by network pharmacology. Methods: Chemical components and targets related to the three traditional Chinese medicine (TCM) herbs were found by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The targets were uploaded to UniProtKB software, then the target genes were mapped with NSCLC genes in the annotated database of human genome (Genecards). The Cytoscape 3.7.0 software was used to construct a chemic-target network, a protein-protein interaction (PPI) network was constructed using the STRING 10.5 software, and the DAVID 6.8 software was used to enrich biological pathways in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results: Totally 23 components and 100 targets related to the three TCM herbs were obtained through screening. PPI network contained 93 targets, key targets involved IL6, TNF, JUN, MYC, CASP3, MAPK8 and PTGS2, etc. There were 43 GO entries, among which 32 were related to biological processes, 6 were related to molecular functions, and 5 were related to cell composition. There were 5 KEGG pathways, involving cancer, IL-17, TNF, estrogen and NF-kappa B. Conclusion: The results of this study preliminarily elucidate the basic pharmacological action and mechanism of Kangai Injection against NSCLC, laying good foundation for further research, and provided new ideas for the development of anti-cancer adjuvant drugs.
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