文章摘要
伏光耀,张小龙,陈洪岩.LC-MS/MS法测定木香槟榔丸中7个有效成分的含量[J].药学与临床研究,2021,29(4):263~266
LC-MS/MS法测定木香槟榔丸中7个有效成分的含量
Determination of 7 Components in Muxiang Binglang Pills by High Performance Liquid Chromatography-Mass Spectrometry
投稿时间:2021-01-28  修订日期:2021-08-13
DOI:
中文关键词: 液质联用法  去氢木香内酯  α-香附酮  氢溴酸槟榔碱  盐酸小檗碱  橙皮苷  大黄素  大黄酚  木香槟榔丸
英文关键词: HPLC-MS/MS  Arecoline hydrobromide  α-Cyperone  Dehydrocostus lactone  Berberine hydrochloride  Hesperidin  Emodin  Chrysophanol  Muxiang Binglang Pills
基金项目:
作者单位E-mail
伏光耀 连云港经济技术开发区市场监督管理局 1601932645@qq.com 
张小龙 江苏食品药品职业技术学院  
陈洪岩 连云港市食品药品检验检测中心 348324009@qq.com 
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中文摘要:
      目的:运用液质联用法测定木香槟榔丸中去氢木香内酯、α-香附酮、氢溴酸槟榔碱、盐酸小檗碱、橙皮苷、大黄素、大黄酚等7个成分的含量。方法:采用液相色谱串联三重四级杆质谱法进行测定,色谱柱为Agilent Poroshell 120 SB C18(2.1 mm×100 mm,2.7 μm),以0.1%甲酸水溶液-乙腈流动相,梯度洗脱,流速为0.3 mL·min-1;离子源为电喷雾离子源(ESI),正负离子模式,采用多反应监测模式(MRM)进行测定。结果:上述7个成分在相应的浓度范围内呈现良好的线性关系;加样回收率为97.94%~101.46%,RSD为1.47%~3.33%(n=6)。结论:该检测方法准确、可靠,可同时测定木香槟榔丸中7个有效成分,为该制剂的质量控制提供了依据。
英文摘要:
      Objective: To develop a high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method for content determination of arecoline hydrobromide, α-cyperone, dehydrocostus lactone, berberine hydrochloride, hesperidin, emodin and chrysophanol in Muxiang Binglang Pills. Methods: The 7 components in Muxiang Binglang Pills were determined by high performance liquid chromatography coupled with triple quadrupole mass spectrometer. An Agilent Poroshell 120 SB C18(2.1 mm × 100 mm, 2.7 μm)column was used. The mobile phase was 0.1% formic acid-acetonitrile with gradient elution and the flow rate was 0.3 mL·min-1. The ion source was from electrospray ionization (ESI). The spectra were recorded under positive and negative ion types with multiple reaction monitoring (MRM) mode. Results: The calibration curves of the 7 components showed good linear relationship in the given concentration ranges. The average recoveries were 97.94%-101.46% with RSD at 1.46%-3.33% (n=6). Conclusion: The method is accurate and reliable. It can be used for the simultaneous determination of the 7 components in the quality control of Muxiang Binglang Pills.
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