文章摘要
杨光,陈淑敏,马灵珍.HPLC一测多评法测定荜铃胃痛颗粒中8种成分的含量[J].药学与临床研究,2021,29(4):259~262
HPLC一测多评法测定荜铃胃痛颗粒中8种成分的含量
Determination of 8 Active Components in Biling Weitong Granules by HPLC-QAMS
投稿时间:2020-05-28  修订日期:2021-08-13
DOI:
中文关键词: 高效液相色谱一测多评法  荜铃胃痛颗粒  吴茱萸碱  吴茱萸次碱  香附烯酮  圆柚酮  α-香附酮  延胡索乙素  去氢紫堇碱  紫堇碱  校正因子
英文关键词: HPLC-QAMS  Biling Weitong granules  Evodiamine  Rutaecarpine  Cyperotundone  Nootkatone  α-Cyperone  Tetrahydropalmatine  Dehydrocorydalin  Corydaline  Correction factor
基金项目:安徽省教育厅高校自然科学研究项目(KJ2017A770)
作者单位E-mail
杨光 郑州颐和医院药学部 zg29897919@sina.com 
陈淑敏 郑州颐和医院药学部  
马灵珍 亳州职业技术学院药学院 zg29897919@sina.com 
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中文摘要:
      目的:建立高效液相色谱一测多评法(HPLC-QAMS)同时测定荜铃胃痛颗粒中延胡索乙素等8种成分的含量。方法:采用高效液相色谱法,色谱柱:Agilent Eclipse C18(250 mm×4.6 mm,5 μm),柱温:30 ℃;流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;流速:0.9 mL·min-1;检测波长分别为215、242和280 nm。以延胡索乙素为内参物,建立其与其他7种成分的校正因子,计算各成分含量。结果:8种成分在各自范围内线性关系良好(r≥0.999 1);平均加样回收率及相对标准偏差(RSD)分别为98.74%(1.13%)、99.62%(0.87%)、100.01%(1.06%)、96.91%(1.17%)、99.35%(1.23%)、98.67%(1.32%)、97.60%(0.94%)、98.42%(0.79%);荜铃胃痛颗粒中8个成分含量的高效液相色谱一测多评法结果与外标法(ESM)测得值无明显差异。结论:建立的一测多评法结果准确,可用于荜铃胃痛颗粒的质量控制。
英文摘要:
      Objective: To establish an HPLC-QAMS method for the determination of 8 active components in Biling Weitong granules. Methods: An HPLC method with an Agilent Eclipse C18 (250 mm × 4.6 mm, 5 μm) column was applied, the mobile phase was gradient acetonitrile-0.1% phosphoric acid aqueous solution, the flow rate was 0.9 mL·min-1, the column temperature was 30 ℃. The detection wavelengths were set at 215, 242 and 280 nm. Using tetrahydropalmatine as the internal standard, correction factors of the other seven constituents were calculated for content determination. Results: The eight components showed good linear relationships within their own ranges (r≥0.999 1), whose average recoveries (RSDs) were 98.74%(1.13%), 99.62%(0.87%), 100.01%(1.06%), 96.91%(1.17%), 99.35%(1.23%), 98.67%(1.32%), 97.60%(0.94%) and 98.42%(0.79%), respectively. No significant difference was found in the quantitative results of the eight components in Biling Weitong granules between the ESM and the HPLC-QAMS methods. Conclusion: The established QAMS method is accurate, which can be used for the quality control of Biling Weitong granules.
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