文章摘要
郭怀宇,陈金锋,谢俊杰,郁冬冬,姜蕾.生血方的鉴别与含量测定[J].药学与临床研究,2022,30(4):311~314
生血方的鉴别与含量测定
Identification and Content Determination of Shengxue Fang
投稿时间:2022-02-08  修订日期:2022-08-20
DOI:
中文关键词: 生血方  高效液相-蒸发光散射检测器  芒柄花素  黄芪甲苷(黄芪皂苷Ⅳ)  方法验证
英文关键词: Shengxue Fang  HPLC-ELSD  Formononetin  Astragaloside IV  Methods validation
基金项目:
作者单位E-mail
郭怀宇 六安市中医院药剂科 guohuaiyu13@163.com 
陈金锋 六安市中医院药剂科  
谢俊杰 六安市中医院药剂科  
郁冬冬 六安市中医院药剂科  
姜蕾 六安市中医院药剂科  
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中文摘要:
      目的:建立生血方的鉴别和含量测定。方法:采用薄层色谱法(TLC)对生血方中黄芪、女贞子和当归进行定性鉴别;采用高效液相色谱法(HPLC-ELSD)对生血方中芒柄花素和黄芪甲苷进行含量测定,流动相为乙腈-水,流速1 mL·min-1;使用蒸发光散射检测器,载气气压0.5 MPa,漂移管温度为100 ℃。结果:在TLC图谱中,斑点清晰,分离度良好,阴性样品无干扰。方中2个成分(芒柄花素和黄芪甲苷)完全分离,峰面积的对数与进样量的对数呈良好的线性关系(r=0.999,0.999 4)。3批样品中黄芪甲苷和芒柄花素的含量分别在0.641 1~0.673 5、0.480 8~0.500 8 mg·g-1。结论:经方法学验证,该方法可用于生血方的质量控制。
英文摘要:
      Objective: To establish a method for identification and content determination for Shengxue Fang. Methods: Astragali Radix, Ligustrum Lucidum and Angelicae Sinensis Radix were identified by TLC qualitatively. The contents of formononetin and astragaloside IV were determined by HPLC-ELSD. The mobile phase of acetonitrile and water was eluted at 1.0 mL·min-1. The detector was ELSD, of which the drift tube temperature was maintained at 100 ℃, and the pressure of nebulizing gas was 0.5 MPa. Results: The TLC spots were clear and the resoluion was good without the interference of negative samples. The HPLC-ELSD results showed that the formononetin and astragaloside IV were completely separated; the regression equation showed good liner relationship between the logarithm of peak areas and the logarithm of contents of each compound (r=0.999, 0.999 4). The contents of formononetin and astragaloside IV in 3 Shengxue Fang samples were 0.6411-0.6735 mg·g-1 and 0.4808-0.5008 mg·g-1, respectively. Conclusion: The established method is validated by methodology, and is suitable for the quality control of Shengxue Fang.
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