文章摘要
戴丽娜,陈泳君,杨颖.HPLC双波长法同时测定桑白皮水提物中6个成分的含量[J].药学与临床研究,2022,30(6):501~504
HPLC双波长法同时测定桑白皮水提物中6个成分的含量
Simultaneous Determination of Six Components in Mori Cortex Water Extracts by an HPLC Dual Wavelength Method
投稿时间:2022-07-19  修订日期:2022-12-17
DOI:
中文关键词: 桑白皮水提物  桑皮苷A  绿原酸  单宁酸  桑根酮D  桑酮G  桑根酮C
英文关键词: Mori Cortex water extract  Mulberroside A  Chlorogenic acid  Tannic acid  Sanggenon D  Kuwanon G  Sanggenon C
基金项目:江苏省市场监督管理局科技计划项目(KJ2022046)
作者单位邮编
戴丽娜 连云港市食品药品检验检测中心 222006
陈泳君 连云港市食品药品检验检测中心 
杨颖 连云港市食品药品检验检测中心 222006
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中文摘要:
      目的:建立HPLC双波长法同时测定桑白皮水提物中桑皮苷A、绿原酸、单宁酸、桑根酮D、桑酮G、桑根酮C等6个主要成分的含量。方法:色谱柱为Phenomenex Gemini C18 (250 mm × 4.6 mm,5 μm);以乙腈为流动相A,0.2%甲酸水溶液为流动相B,梯度洗脱;检测波长:270 nm(单宁酸、桑根酮D、桑酮G、桑根酮C)、320 nm(桑皮苷A、绿原酸);柱温:30℃;流速:1.0 mL·min-1;进样量:20 μL。结果:桑皮苷A、绿原酸、单宁酸、桑根酮D、桑酮G、桑根酮C分别在3.24 ~ 647.20 μg·mL-1、3.22 ~ 643.60 μg·mL-1、2.50 ~ 588.80 μg·mL-1、3.28 ~ 656.80 μg·mL-1、3.19 ~ 637.20 μg·mL-1、3.10 ~ 619.60 μg·mL-1质量浓度范围内与峰面积线性关系良好(r > 0.999);加样平均回收率分别为99.18%、99.06%、98.96%、98.76%、98.99%、98.90%(n = 9),其RSD分别为0.45%、0.33%、0.59%、0.36%、0.47%、0.49%。结论:该方法的准确度、重复性、耐用性均良好,适合用于桑白皮水提物中桑皮苷A等6个主要成分的定量测定。
英文摘要:
      Objective: To establish an HPLC dual wavelength method for determination of six components in Mori Cortex water extracts. Method: HPLC was conducted on a Phenomenex Gemini C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was constituted of acetonitrile(A)-0.2% formic acid (B) in a gradient mode. The detection wavelength was set at 270 nm for tannic acid, sanggenon D, kuwanon G and sanggenon C, and 320 nm for mulberroside A and chlorogenic acid. The flow rate was 1.0 mL·min-1. The column temperature was 30°C. The injection volume was 20 μL. Results: Mulberroside A, chlorogenic acid, tannic acid, sanggenon D, kuwanon G and sanggenon C showed good linear relationships within the ranges of 3.24- 647.20 μg·mL-1, 3.22 - 643.60 μg·mL-1, 2.50 - 588.80 μg·mL-1, 3.28 - 656.80 μg·mL-1, 3.19 - 637.20 μg·mL-1 and 3.10 - 619.60 μg·mL-1, respectively (r > 0.999 ). Recovery rates were 99.18%, 99.06%, 98.96%, 98.76%, 98.99% and 98.90% (n = 9), respectively. RSD were 0.45%, 0.33%, 0.59%, 0.36%, 0.47% and 0.49%, respectively. Conclusion: The method can carry out the qualitative and quantitative analysis with high accuracy, repeatability and durability, and it is suitable for quantitative analysis of the six components in Mori Cortex water extracts.
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